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Table 6. Metabolite Profiling Technology Development Roadmap (acronyms)

Technology Objectives

Research, Design, Development

Demonstration: Pilots, Modular Deployment

Integration, Production Deployment

Products

High-Throughput Metabolite Profiling

LC/MS and NMR methods for metabolite discovery

Sample processing

Data processing

Analysis, quantitation, QA/QC

Define requirements:

  • Workflow processes
  • Detection limits, reproducibility, dynamic range
  • Lab automation, robotics, QC
  • Robust LC-NMR techniques
  • Hardware, software, instrumentation

Develop methods:

  • Identification and quantitation
  • QA/QC with metrics
  • Sample processing

Establish pilot metabolite-profiling pipeline

Optimize, scale up

Develop improved standards, protocols, costs

Establish high-throughput pipeline based on defined requirements, standards, protocols, costs

High-quality, comprehensive, metabolite-profiling data linked to experiment archive and culture and sampling data

To develop and incorporate the necessary technologies and methods into a high-throughput production environment, a phased process will be followed as described in this roadmap. The process includes research, design, and development; modular and pilot-scale deployment; and final integration and scaleup into operational procedures.