DOE Genomes
Human Genome Project Information Genomics:GTL DOE Microbial Genomics home
-

Skip navigation links

Close Window

Table 3. Transcriptome Analysis Technology Development Roadmap (acronyms)

Technology Objectives

Research, Design, Development

Demonstration: Pilots and Modular Deployment

Integration and Production Deployment

Products

High-Throughput Gene-Expression Profiling

Sample processing

Data processing

Quantitation

QA/QC

Define:

  • Workflow processes
  • Improved detection limits, reproducibility, dynamic range
  • Hardware, software
  • Lab automation, robotics
  • QC instrumentation, processes

Develop:

  • Multipurpose, multiorganism array platform
  • In vivo testing platforms
  • Expression database
  • Commercial array applications

Expression pipeline optimization, scaleup:

  • Improved standards, protocols, costs

Pilot:

  • Array processing pipeline
  • Expression database
  • In vivo testing pipeline

Establish high-throughput pipeline based on defined requirements, standards, protocols, costs, and adopted industry standards:

  • Array processing pipeline
  • Expression-experiment database
  • In vivo expression-testing pipeline

High-quality, comprehensive expression data linked to experiment archive and culture and sampling data

To develop and incorporate the necessary technologies and methods into a high-throughput production environment, a phased process will be followed as described in this roadmap. The process includes research, design, and development; modular and pilot-scale deployment; and final integration and scaleup into operational procedures.