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Table 5. Technology Development Roadmap for Complex Validation and Characterization (acronyms)

Technology Objectives

Research, Design, and Development

Demonstration:
Pilots and Modular Deployment

Integration and Production Deployment

Facility Outputs

Develop technologies for complex validation and characterization

Analysis of complexes in vitro and in vivo:

  • Data processing
  • Data archiving

Develop:

  • In vivo imaging for validation and spatial and temporal studies
  • New labels for optical microscopy
  • Multimodal imaging approaches
  • Automated image acquisition
  • High-throughput image analysis
  • Improved spatial resolution
  • Environmental sample-manipulation techniques

Evaluate commercial hardware, software, and instrumentation

High-throughput EM

High-throughput optical methods

Image-analysis software

Automated sample acquisition

Multimodal imaging

Automate image acquisition

Automate data analysis

Scale up acquisition and analysis

Establish database

Evaluate and incorporate new technologies

Data and characterizations:

  • Existence of complexes
  • Dynamic spatial relationships of proteins and other macromolecules in complexes
  • Local chemical and physical environment of complexes in cells

To develop and incorporate the necessary technologies and methods into a high-throughput production environment, a phased process will be followed as described in this roadmap. The process includes research, design, and development; modular and pilot-scale deployment; and final integration and scaleup into operational procedures.