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Table 1. Technology Development Roadmap for Cell Growth and Processing (acronyms)

Technology Objectives

Research, Design, and Development

Demonstration:
Pilots and Modular Deployment

Integration and Production Deployment

Facility Outputs

Develop technologies for protein machine production: Cell growth and processing

Cultivation systems:

  • Modified for endogenous protein isolations
  • Wild type for exogenous complex isolations

Define conditions to express and process active molecular machines

Develop methods:

  • Reproducible growth, real-time monitoring, sampling
  • Novel culture approaches
  • High-throughput controlled fermentations
  • Sample archive documentation
  • Functional assays for unknown isolated molecular machines

Evaluate commercial systems

Controlled cell growth, processing:

  • Modified cultures for endogenous complex isolation
  • Wild-type microbes for exogenous complex isolation
  • Large numbers of microbial clones with encoded tags

Database development

Controlled bioreactors for cellular imaging

Automation and standardization

Standards, protocols, costs, QA/QC refinements

Evaluation, incorporation of new technologies

Development of methods for microbes and machines requiring specialized conditions

Establish high-throughput pipeline based on defined requirements, standards, protocols, costs

Scale up parallel processes for multiple organisms

Evaluate and incorporate new technologies

Use parallel processes for scaleup

Production of well-defined microbial samples for extraction and characterization of active molecular machines

Database from controlled cell growth with analysis of protein complexes and associated biocompounds

Well-managed biosample archive

Protocols

To develop and incorporate the necessary technologies and methods into a high-throughput production environment, a phased process will be followed as described in this roadmap. The process includes research, design, and development; modular and pilot-scale deployment; and final integration and scaleup into operational procedures.