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Objectives and Subtopics |
Research |
Pilots |
Production |
Products |
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Protein Production Small soluble proteins |
Protocol refinement Optimization for cost-effectiveness |
Scale up to 2 k/yr Protocol standards QA standards |
Scale up to 25k/yr |
Multiple forms of proteins Protein chips Protocols |
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Protein Production Membrane proteins |
Detergents Refolding Novel expression systems Cell-free expression Chemical synthesis Domain identification Domain expression |
Evaluate/validate expression systems Protocol standards QA standards |
Automate Scale up |
Multiple forms of proteins Protocols |
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Protein Production Multiple domain proteins Proteins with fusion tags |
Refolding Novel expression systems Cell-free expression Chemical synthesis Domain identification Domain expression |
Evaluate/validate expression systems Protocol standards QA standards |
Automate Scale up |
Multiple forms of proteins Individual protein domains Protein chips Protocols |
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Protein Production Multiprotein complexes (when needed for co-expression or stabilization and storage) |
Binding-partner identification Refolding Novel expression systems Cell-free expression |
Evaluate/validate coexpression systems Protocol standards QA standards |
Automate Scale up |
Multiple forms of proteins Protocols ID binding partners |
To develop and incorporate the necessary technologies and methods into a high-throughput production environment, a phased process will be followed as described in this roadmap. The process includes research, design, and development; modular and pilot-scale deployment; and final integration and scaleup into operational procedures.
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Genomic Science (formerly Genomics:GTL) is a program of the
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Base URL: http://genomicscience.energy.gov
Last modified: Wednesday, July 11, 2007